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Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Recombinant antibody, PBS Only

Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2P21

Cat no : 80115-1-PBS

Synonyms

MGF, Phospho-STAT5A (Tyr694)/STAT5B (Tyr699), p-STAT5B T699, STAT5, STAT5A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Recombinan (80115-1-RR)

    WB analysis using 80115-1-RR (same clone as 80115-1-PBS)

    Non-treated TF-1 and GM-CSF (HZ-1002) treated TF-1 cells were subjected to SDS PAGE followed by western blot with 80115-1-RR (Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control. This data was developed using the same antibody clone with 80115-1-PBS in a different storage buffer formulation.

Product Information

80115-1-PBS targets Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name signal transducer and activator of transcription 5A
Calculated Molecular Weight 794 aa, 92 kDa
Observed Molecular Weight90-95 kDa
GenBank Accession NumberBC027036
Gene Symbol STAT5A
Gene ID (NCBI) 6776
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells.