Phospho-STAT5A (Ser726) Recombinant antibody

Phospho-STAT5A (Ser726) Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240709D6

Cat no : 80138-2-RR

Synonyms

p STAT5A Ser726, p STAT5A (Ser726), p STAT5A, p STAT5 (Ser726), Phospho STAT5 (Ser726)

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Validation Data Gallery

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  WB analysis using 80138-2-RR

  Non-treated and GM-CSF treated TF-1 cells were subjected to SDS PAGE followed by western blot with 80138-2-RR (Phospho-STAT5A (Ser726) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

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  IF Staining of TF-1 using 80138-2-RR

  Immunofluorescent analysis of (4% PFA) fixed GM-CSF treated TF-1 cells using Phospho-STAT5A (Ser726) antibody (80138-2-RR, Clone: 240709D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of TF-1 using 80138-2-RR

  1x10^6 untreated TF-1 cells (dash lines) and 100 nM Calyculin A (30 minutes) treated TF-1 cells (full lines) were intracellularly stained with 0.4 μg Phospho-STAT5A (Ser726) Recombinant antibody (80138-2-RR, Clone: 240709D6, red) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR005). Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9, blue) was parallel stained as control. Cells were fixed with 4% PFA.

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Tested Applications

• Positive WB detected in: GM-CSF treated TF-1 cells
• Positive IF/ICC detected in: GM-CSF treated TF-1 cells
• Positive FC (Intra) detected in: 100 nM Calyculin A (30 minutes) treated TF-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80138-2-RR targets Phospho-STAT5A (Ser726) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: signal transducer and activator of transcription 5A
• Calculated Molecular Weight: 794 aa, 92 kDa
• Observed Molecular Weight: 90-95 kDa
• GenBank Accession Number: BC027036
• Gene Symbol: STAT5A
• Gene ID (NCBI): 6776
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STAT5 proteins consisting of STAT5A and STAT5B are the main targets of IL-3 signaling. Upon cytokine stimulation, JAK2 phosphorylates STAT5 and the phosphorylated STAT5 proteins dimerize and translocate into the nucleus, where, by binding DNA, they activate target genes including c-fos. In addition to this JAK-catalyzed tyrosine phosphorylation, STAT5 may undergo serine phosphorylation in the carboxy-terminal P(M)SP site in response to prolactin. (PMID: 15795318)