Phospho-STAT5A (Ser726) Recombinant antibody

Phospho-STAT5A (Ser726) Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240709D6

Cat no : 80138-2-RR

Synonyms

p STAT5A Ser726, p STAT5A (Ser726), p STAT5A, p STAT5 (Ser726), Phospho STAT5 (Ser726)

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Validation Data Gallery

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  WB analysis using 80138-2-RR

  Non-treated and GM-CSF treated TF-1 cells were subjected to SDS PAGE followed by western blot with 80138-2-RR (Phospho-STAT5A (Ser726) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

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Tested Applications

• Positive WB detected in: GM-CSF treated TF-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80138-2-RR targets Phospho-STAT5A (Ser726) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: signal transducer and activator of transcription 5A
• Calculated Molecular Weight: 794 aa, 92 kDa
• Observed Molecular Weight: 90-95 kDa
• GenBank Accession Number: BC027036
• Gene Symbol: STAT5A
• Gene ID (NCBI): 6776
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STAT5 proteins consisting of STAT5A and STAT5B are the main targets of IL-3 signaling. Upon cytokine stimulation, JAK2 phosphorylates STAT5 and the phosphorylated STAT5 proteins dimerize and translocate into the nucleus, where, by binding DNA, they activate target genes including c-fos. In addition to this JAK-catalyzed tyrosine phosphorylation, STAT5 may undergo serine phosphorylation in the carboxy-terminal P(M)SP site in response to prolactin. (PMID: 15795318)