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CoraLite® Plus 488-conjugated Phospho-RPS6KA1 (Ser380) Recombinant antibody

Phospho-RPS6KA1 (Ser380) Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

7F23

Cat no : CL488-80108

Synonyms

RPS6KA1, MAPKAP kinase 1a, MAPK-activated protein kinase 1a, MAP kinase-activated protein kinase 1a, HU 1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 488-conjugated Phospho-RPS6KA1 (Ser (CL488-80108)

    FC experiment of Jurkat using CL488-80108

    1X10^6 Jurkat cells untreated (dashed lines) or treated with TPA (red) were intracellularly stained with 0.13 ug CoraLite® Plus 488 Anti-Human Phospho-RPS6KA1 (Ser380) (CL488-80108, Clone:7F23), or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Tested Applications

Positive FC (Intra) detected inTPA treated Jurkat cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-80108 targets Phospho-RPS6KA1 (Ser380) in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name ribosomal protein S6 kinase, 90kDa, polypeptide 1
Calculated Molecular Weight 735 aa, 83 kDa
Observed Molecular Weight83-90 kDa
GenBank Accession NumberBC014966
Gene Symbol RPS6KA1
Gene ID (NCBI) 6195
RRIDAB_2919572
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. It has been reported that the phosphorylation of RPS6KA1 at Ser380 is involved in autophosphorylation by activation of the C-terminal kinase domain. (PMID: 16458888, 30813401, 17259979, 10679322)