Phospho-RIPK1 (Ser161) Monoclonal antibody, PBS Only

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  WB analysis using 66854-1-Ig (same clone as 66854-1-PBS)

  Non-treated HEK-293T and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 66854-1-Ig (Phospho-RIPK1 (Ser161) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 66854-1-PBS in a different storage buffer formulation.

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  WB analysis using 66854-1-Ig (same clone as 66854-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66854-1-Ig (Phospho-RIPK1 (Ser161) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was untreated (left) or treated with Lambda Protein Phosphatase (λ-PPase, 500U, right) at 37℃ for 1 hours before blocking. This data was developed using the same antibody clone with 66854-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 66854-1-Ig (same clone as 66854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66854-1-Ig (RIPK1 phospho S161 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66854-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 66854-1-Ig (same clone as 66854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66854-1-Ig (RIPK1 phospho S161 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66854-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66854-1-Ig (same clone as 66854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66854-1-Ig (RIPK1 phospho S161 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66854-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66854-1-Ig (same clone as 66854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66854-1-Ig (RIPK1 phospho S161 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66854-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 66854-1-Ig (same clone as 66854-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Phospho-RIPK1 (Ser161) antibody (66854-1-Ig, Clone: 1B2G1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66854-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66854-1-Ig (same clone as 66854-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-RIPK1 (Ser161) antibody (66854-1-Ig, Clone: 1B2G1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66854-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293T using 66854-1-Ig (same clone as 66854-1-PBS)

  1X10^6 Calyculin A treated HEK-293T cells were intracellularly stained with 0.13 ug Anti-Human Phospho-RIPK1 (Ser161) (66854-1-Ig, Clone:1B2G1) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 66854-1-PBS in a different storage buffer formulation.

Phospho-RIPK1 (Ser161) Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 66854-1-PBS

Clone No.1B2G1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

p RIPK1, p RIPK1 (Ser161), p RIPK1 Ser161, Phospho RIPK1, Phospho RIPK1 (Ser161)

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Product Information

66854-1-PBS targets Phospho-RIPK1 (Ser161) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: receptor (TNFRSF)-interacting serine-threonine kinase 1
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 74 kDa, 45 kDa
• GenBank Accession Number: NM_003804
• Gene Symbol: RIPK1
• Gene ID (NCBI): 8737
• RRID: AB_2882194
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q13546
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397).