Phospho-RASGRF1 (Ser927) Recombinant antibody

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  WB analysis of HeLa using 82309-1-RR

  Non-treated and Forskolin treated HeLa cells were subjected to SDS PAGE followed by western blot with 82309-1-RR (Phospho-RASGRF1 (Ser927) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR (66888-1-Ig) as loading control.

Phospho-RASGRF1 (Ser927) Recombinant Antibody for WB, ELISA

Cat No. 82309-1-RR

Clone No.5K3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

p RASGRF1, p RASGRF1 (Ser927), p RASGRF1 Ser927, Phospho RASGRF1, Phospho RASGRF1 (Ser927)

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Tested Applications

• Positive WB detected in: Forskolin treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82309-1-RR targets Phospho-RASGRF1 (Ser927) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: Ras protein-specific guanine nucleotide-releasing factor 1
• Calculated Molecular Weight: 134 kDa
• Observed Molecular Weight: 145 kDa
• GenBank Accession Number: BC040275
• Gene Symbol: RASGRF1
• Gene ID (NCBI): 5923
• RRID: AB_3085358
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13972
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The RASGRF1 exchange factor, which is also known as CDC25 in Saccharomyces cerevisiae, promotes the exchange of Ras-bound GDP by GTP, is known to play central roles in pathways of cellular growth and differentiation. The serine residue at position 916 in mouse Ras-GRF1 is a PKA phosphorylation site, and the corresponding human residue is serine 927. It's reported that phosphorylation of serine 916 is necessary but not sufficient for maximal activation of mouse RASGRF1. The calculated MW of non-phosphorylated RASGRF1 is 145 kDa. 82309-1-RR can detect a band around 145 kDa, and can detect the increasing phosphorylation level after Forskolin treated.(PMID:15853814, 10601308)