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Phospho-RASGRF1 (Ser927) Recombinant antibody, PBS Only

Phospho-RASGRF1 (Ser927) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5K3

Cat no : 82309-1-PBS

Synonyms

RASGRF1, GRF55, GRF1, GNRP, CDC25L

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Phospho-RASGRF1 (Ser927) Recombinant antibody (82309-1-RR)

    WB analysis of HeLa using 82309-1-RR (same clone as 82309-1-PBS)

    Non-treated and Forskolin treated HeLa cells were subjected to SDS PAGE followed by western blot with 82309-1-RR (Phospho-RASGRF1 (Ser927) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR (66888-1-Ig) as loading control. This data was developed using the same antibody clone with 82309-1-PBS in a different storage buffer formulation.

Product Information

82309-1-PBS targets Phospho-RASGRF1 (Ser927) in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name Ras protein-specific guanine nucleotide-releasing factor 1
Calculated Molecular Weight 134 kDa
Observed Molecular Weight145 kDa
GenBank Accession NumberBC040275
Gene Symbol RASGRF1
Gene ID (NCBI) 5923
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The RASGRF1 exchange factor, which is also known as CDC25 in Saccharomyces cerevisiae, promotes the exchange of Ras-bound GDP by GTP, is known to play central roles in pathways of cellular growth and differentiation. The serine residue at position 916 in mouse Ras-GRF1 is a PKA phosphorylation site, and the corresponding human residue is serine 927. It's reported that phosphorylation of serine 916 is necessary but not sufficient for maximal activation of mouse RASGRF1. The calculated MW of non-phosphorylated RASGRF1 is 145 kDa. 82309-1-RR can detect a band around 145 kDa, and can detect the increasing phosphorylation level after Forskolin treated.(PMID:15853814, 10601308)