Phospho-Paxillin (Tyr118) Recombinant antibody

• 

  WB analysis using 81180-2-RR

  Non-treated and pervanadate treated HeLa cells were subjected to SDS PAGE followed by western blot with 81180-2-RR (Phospho-Paxillin (Tyr118) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

Phospho-Paxillin (Tyr118) Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 81180-2-RR

Clone No.241063E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: pervanadate treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81180-2-RR targets Phospho-Paxillin (Tyr118) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: paxillin
• Calculated Molecular Weight: 591 aa, 65 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC136787
• Gene Symbol: Paxillin
• Gene ID (NCBI): 5829
• RRID: AB_3670493
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P49023
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Paxillin (p68-kD) is one of the integrin assembly proteins and can interact directly with several integrin assembly proteins, including vinculin, talin, β1 integrin, focal adhesion kinase (FAK), c-Src and Csk. Integrin-mediated tyrosine phosphorylation also enables paxillin to interact with various signaling molecules: tyrosine residues (Y) 31 and 118 being especially predominant targets of phosphorylation by kinases and creating binding sites for the SH2 domain of adaptor protein Crk. Thus, paxillin plays apivotal role in cell adhesion, migration and further oncogenic transformation. (PMID: 11774284)