Phospho-Paxillin (Tyr118) Recombinant antibody, PBS Only

• 

  WB analysis using 81180-2-RR (same clone as 81180-2-PBS)

  Non-treated and pervanadate treated HeLa cells were subjected to SDS PAGE followed by western blot with 81180-2-RR (Phospho-Paxillin (Tyr118) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81180-2-PBS in a different storage buffer formulation.

Phospho-Paxillin (Tyr118) Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 81180-2-PBS

Clone No.241063E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

---

Product Information

81180-2-PBS targets Phospho-Paxillin (Tyr118) in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: paxillin
• Calculated Molecular Weight: 591 aa, 65 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC136787
• Gene Symbol: Paxillin
• Gene ID (NCBI): 5829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P49023
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Paxillin (p68-kD) is one of the integrin assembly proteins and can interact directly with several integrin assembly proteins, including vinculin, talin, β1 integrin, focal adhesion kinase (FAK), c-Src and Csk. Integrin-mediated tyrosine phosphorylation also enables paxillin to interact with various signaling molecules: tyrosine residues (Y) 31 and 118 being especially predominant targets of phosphorylation by kinases and creating binding sites for the SH2 domain of adaptor protein Crk. Thus, paxillin plays apivotal role in cell adhesion, migration and further oncogenic transformation. (PMID: 11774284)