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Phospho-Paxillin (Tyr118) Recombinant antibody, PBS Only

Phospho-Paxillin (Tyr118) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241063E10

Cat no : 81180-2-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-Paxillin (Tyr118) Recombinant antibody (81180-2-RR)

    WB analysis using 81180-2-RR (same clone as 81180-2-PBS)

    Non-treated and pervanadate treated HeLa cells were subjected to SDS PAGE followed by western blot with 81180-2-RR (Phospho-Paxillin (Tyr118) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81180-2-PBS in a different storage buffer formulation.

Product Information

81180-2-PBS targets Phospho-Paxillin (Tyr118) in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name paxillin
Calculated Molecular Weight 591 aa, 65 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC136787
Gene Symbol Paxillin
Gene ID (NCBI) 5829
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Paxillin (p68-kD) is one of the integrin assembly proteins and can interact directly with several integrin assembly proteins, including vinculin, talin, β1 integrin, focal adhesion kinase (FAK), c-Src and Csk. Integrin-mediated tyrosine phosphorylation also enables paxillin to interact with various signaling molecules: tyrosine residues (Y) 31 and 118 being especially predominant targets of phosphorylation by kinases and creating binding sites for the SH2 domain of adaptor protein Crk. Thus, paxillin plays apivotal role in cell adhesion, migration and further oncogenic transformation. (PMID: 11774284)