Phospho-P53 (Ser392) Recombinant antibody

Phospho-P53 (Ser392) Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241575F6

Cat no : 80077-2-RR

Synonyms

Phospho P53 (Ser392), Phospho P53, P53 (Ser392), p P53 Ser392, p P53 (Ser392)

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Validation Data Gallery

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  WB analysis of HEK-293 using 80077-2-RR

  Phosphatase inhibitor treated or λ phosphatase treated or untreated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80077-2-RR (Phospho-P53 (Ser392) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 80077-2-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Phospho-P53 (Ser392) antibody (80077-2-RR, Clone: 241575F6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 80077-2-RR

  1x10^6 λ phosphatase treated HeLa cells were intracellularly stained with 0.2 ug Phospho-P53 (Ser392) Recombinant Antibody (80077-2-RR, Clone:241575F6)(red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9)(blue) and PE-conjugated Goat Anti-Rabbit IgG. 1x10^6 untreated HeLa cells were intracellularly stained with 0.2 ug Phospho-P53 (Ser392) Recombinant Antibody (80077-2-RR, Clone:241575F6) and PE-conjugated Goat Anti-Rabbit IgG(black). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Tested Applications

• Positive WB detected in: λ phosphatase treated HEK-293 cells
• Positive IF/ICC detected in: A431 cells
• Positive FC (Intra) detected in: λ phosphatase treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80077-2-RR targets Phospho-P53 (Ser392) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: tumor protein p53
• Calculated Molecular Weight: 44 kDa
• GenBank Accession Number: BC003596
• Gene Symbol: P53
• Gene ID (NCBI): 7157
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

P53 is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683)