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CoraLite®555-conjugated Phospho-P53 (Ser15) Monoclonal antibody

Phospho-P53 (Ser15) Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1H6G1

Cat no : CL555-67826

Synonyms

TP53, P53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HT-29 cells using CoraLite®555-conjugated Phospho-P53 (Ser15) Monocl (CL555-67826)

    FC experiment of HT-29 using CL555-67826

    1X10^6 UV treated HT-29 cells were intracellularly stained with 0.25 ug CoraLite®555 Anti-Human Phospho-P53 (Ser15) (CL555-67826, Clone:1H6G1) (red), or 0.25 ug CoraLite® Plus 555 Mouse IgG1 Isotype Control (1F8D3) (CL555-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Tested Applications

Positive FC (Intra) detected inUV treated HT-29 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-67826 targets Phospho-P53 (Ser15) in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name tumor protein p53
Calculated Molecular Weight 44 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC003596
Gene Symbol P53
Gene ID (NCBI) 7157
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)