Phospho-P53 (Ser15) Monoclonal antibody

Phospho-P53 (Ser15) Monoclonal Antibody for WB, IF, FC, ELISA

Host / Isotype

Mouse / IgG1


Human and More (4)







Cat no : 67826-1-Ig


Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phospho-P53 (Ser15), Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

Tested Applications

Positive WB detected inCalyculin A treated HT-29 cells, HT-29 cells, HEK-293T cells, HEK-293T cells, Etoposide treated HT-29 cells
Positive IF detected inetoposide treated HT-29 cells
Positive FC detected inUV treated HT-29 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67826-1-Ig targets Phospho-P53 (Ser15) in WB, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name tumor protein p53
Calculated Molecular Weight 44 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC003596
Gene Symbol TP53
Gene ID (NCBI) 7157
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)


Product Specific Protocols
WB protocol for Phospho-P53 (Ser15) antibody 67826-1-IgDownload protocol
IF protocol for Phospho-P53 (Ser15) antibody 67826-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Virol

Hepatitis B doubly spliced protein (HBDSP) promotes hepatocellular carcinoma cell apoptosis via ETS1/GATA2/YY1-mediated p53 transcription

Authors - Xiazhen Xu

Food Chem Toxicol

Enhanced glutathione production protects against zearalenone-induced oxidative stress and ferroptosis in female reproductive system

Authors - Wei Fu

Front Pharmacol

A New Chalcone Derivative C49 Reverses Doxorubicin Resistance in MCF-7/DOX Cells by Inhibiting P-Glycoprotein Expression.

Authors - Ting Wang

Toxicol Lett

The mechanism of rh-endostatin-induced cardiotoxicity and its protection by dihydromyricetin[in vivo/in vitro, C57BL/6 mice, AC16 and hiPSC-CMs]

Authors - Xiaoran Guan

World Neurosurg

N-acetylserotonin protects rat nucleus pulposus cells against oxidative stress injury by activating the PI3K/AKT signaling pathway

Authors - Wang Yidian

Front Pharmacol

Simultaneous suppression of PKM2 and PHGDH elicits synergistic anti-cancer effect in NSCLC

Authors - Kaixuan Wang