Phospho-MYL9 (Thr19/Ser20) Recombinant antibody, PBS Only

Phospho-MYL9 (Thr19/Ser20) Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G10

Cat no : 82717-1-PBS

Synonyms

MYL9, MLC-2C, MLC2, 1G10

---

Validation Data Gallery

• 

  WB analysis using 82717-1-RR (same clone as 82717-1-PBS)

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82717-1-RR (Phospho-MYL9 (Thr19/Ser20) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control. This data was developed using the same antibody clone with 82717-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 82717-1-RR (same clone as 82717-1-PBS)

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82717-1-RR (Phospho-MYL9 (Thr19/Ser20) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control. This data was developed using the same antibody clone with 82717-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 82717-1-RR (same clone as 82717-1-PBS)

  1x10^6 Calyculin A treated HeLa cells were intracellularly stained with 0.25 ug Phospho-MYL9 (Thr19/Ser20) Recombinant antibody (82717-1-RR, Clone:1G10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 82717-1-PBS in a different storage buffer formulation.

---

This antibody, also known as Thr18/Ser19, is the site where the initiator methionine is removed.

Product Information

82717-1-PBS targets Phospho-MYL9 (Thr19/Ser20) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin, light chain 9, regulatory
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 19-20 kDa
• GenBank Accession Number: BC002648
• Gene Symbol: MYL9
• Gene ID (NCBI): 10398
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Myosin regulatory light polypeptide 9 (MYL9), also known as MLC2, belongs to the myosin regulatory subunits. It plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation at Thr19 and Ser20. Implicated in cytokinesis, receptor capping, and cell locomotion (PMID:11942626, PMID:2526655). Some studies have demonstrated that MYL9 may play important roles in various human cancers. The expression and phosphorylation of MYL9 (Thr19/Ser20) may be increased in human breast (PMID: 22144583) and liver cancers (PMID: 18648664), while decreased in human colon (PMID: 22752057) and bladder cancers (PMID: 21139803). MYL9 was the only gene differentially expressed in the aged versus young injured arteries in the rat smooth muscle cell layers (PMID:22003410).