Phospho-MST1 (Thr183)/MST2 (Thr180) Recombinant antibody

Phospho-MST1 (Thr183)/MST2 (Thr180) Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

1P6

Cat no : 80093-1-RR

Synonyms

MST1, MST2, STK4, STK4/MST1, MST 2

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Validation Data Gallery

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  WB analysis using 80093-1-RR

  Non-treated Ramos and Jurkat and Staurosporine treated Ramos and Jurkat cells were subjected to SDS PAGE followed by western blot with 80093-1-RR (Phospho-MST1 (Thr183)/MST2 (Thr180) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 80093-1-RR

  Non-treated HeLa and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80093-1-RR (Phospho-MST1 (Thr183)/MST2 (Thr180) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 80093-1-RR

  1X10^6 Calyculin A treated HeLa cells were intracellularly stained with 0.25 ug Anti-Human Phospho-MST1 (Thr183)/MST2 (Thr180) (80093-1-RR, Clone:1P6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, Calyculin A treated HeLa cells, Staurosporine treated Ramos cells, Staurosporine treated Jurkat cells
• Positive FC (Intra) detected in: Calyculin A treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

80093-1-RR targets Phospho-MST1 (Thr183)/MST2 (Thr180) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: serine/threonine kinase 4
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC005231
• Gene Symbol: MST1/MST2
• Gene ID (NCBI): 6789
• RRID: AB_2918864
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases . STK3/4 and LATS1/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the STK3/4 and LATS1/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors. Lines of evidence have indicated that dysregulation or loss of STK4/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. STK4 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of STK4 at Thr183 (Thr180 in STK3) in the activation loop is a key activation mechanism for STK4/3 because phosphorylation of Thr183/180 causes the cleavage of STK4 by caspases under apoptotic conditions. The caspase-cleavage results in a more active STK4 protein (STK4-N, an amino-terminally truncated STK4), which localizes into the nucleus and induces apoptosis through histone modifications and chromatin condensations.

Publications

Species	Application	Title
mouse	WB	Ecotoxicol Environ Saf; Role of the hippo signaling pathway in the extracellular matrix degradation of chondrocytes induced by fluoride exposure; Authors - Fang-fang Yu
mouse	WB	J Hazard Mater; Prenatal and postnatal exposure to polystyrene microplastics induces testis developmental disorder and affects male fertility in mice; Authors - Tianxin Zhao
mouse	WB	Nat Commun; KK-LC-1 as a therapeutic target to eliminate ALDH+ stem cells in triple negative breast cancer; Authors - Jiawen Bu
human	WB	Exp Eye Res; Targeting mechanics-induced trabecular meshwork dysfunction through YAP-TGFβ Ameliorates high myopia-induced ocular hypertension; Authors - Qianwen Bu
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Hippo pathway activated by circulating reactive oxygen species mediates cardiac diastolic dysfunction after acute kidney injury; Authors - Xiao Han