Phospho-MST1 (Thr183)/MST2 (Thr180) Recombinant antibody, PBS Only

Phospho-MST1 (Thr183)/MST2 (Thr180) Uni-rAb^TM Recombinant Antibody for Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1P6

Cat no : 80093-1-PBS

Synonyms

MST1, MST2, STK4, STK4/MST1, MST 2

---

Validation Data Gallery

• 

  WB analysis using 80093-1-RR (same clone as 80093-1-PBS)

  Non-treated Ramos and Jurkat and Staurosporine treated Ramos and Jurkat cells were subjected to SDS PAGE followed by western blot with 80093-1-RR (Phospho-MST1 (Thr183)/MST2 (Thr180) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80093-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80093-1-RR (same clone as 80093-1-PBS)

  Non-treated HeLa and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80093-1-RR (Phospho-MST1 (Thr183)/MST2 (Thr180) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80093-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 80093-1-RR (same clone as 80093-1-PBS)

  1X10^6 Calyculin A treated HeLa cells were intracellularly stained with 0.25 ug Anti-Human Phospho-MST1 (Thr183)/MST2 (Thr180) (80093-1-RR, Clone:1P6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80093-1-PBS in a different storage buffer formulation.

---

Product Information

80093-1-PBS targets Phospho-MST1 (Thr183)/MST2 (Thr180) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: serine/threonine kinase 4
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC005231
• Gene Symbol: MST1
• Gene ID (NCBI): 6789
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Mammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases . STK3/4 and LATS1/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the STK3/4 and LATS1/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors. Lines of evidence have indicated that dysregulation or loss of STK4/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. STK4 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of STK4 at Thr183 (Thr180 in STK3) in the activation loop is a key activation mechanism for STK4/3 because phosphorylation of Thr183/180 causes the cleavage of STK4 by caspases under apoptotic conditions. The caspase-cleavage results in a more active STK4 protein (STK4-N, an amino-terminally truncated STK4), which localizes into the nucleus and induces apoptosis through histone modifications and chromatin condensations.