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Phospho-JUN (Thr91/93) Recombinant antibody, PBS Only

Phospho-JUN (Thr91/93) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241983H8

Cat no : 81667-2-PBS

Synonyms

AP1, AP 1, JUN, c Jun, Activator protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-JUN (Thr91/93) Recombinant antibody (81667-2-RR)

    WB analysis using 81667-2-RR (same clone as 81667-2-PBS)

    Non-treated NIH/3T3 cells and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81667-2-RR (Phospho-JUN (Thr91/93) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81667-2-PBS in a different storage buffer formulation.

Product Information

81667-2-PBS targets Phospho-JUN (Thr91/93) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name jun oncogene
Calculated Molecular Weight 331 aa, 36 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC068522
Gene Symbol JUN
Gene ID (NCBI) 3725
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

JUN is also named as c-Jun and AP1, belongs to the bZIP family and Jun subfamily. JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis.The phosphorylation of c-Jun by Plk3 immunoprecipitates was not altered by the pre-removal of JNK from the cell lysates. In addition, the effect of UV irradiation-induced phosphorylation of c-Jun and apoptosis were not significantly affected by knockdown of JNK mRNA. (PMID: 22180088, PMID: 17804415)