Phospho-JUN (Ser73) Recombinant antibody

Phospho-JUN (Ser73) Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

4A18

Cat no : 80086-1-RR

Synonyms

AP1, AP 1, JUN, c Jun, Activator protein 1

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Validation Data Gallery

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  WB analysis using 80086-1-RR

  Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80086-1-RR (Phospho-JUN (Ser73) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody (66009-1-Ig) as loading control.

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  IF Staining of HeLa using 80086-1-RR

  Immunofluorescent analysis of (4% PFA) fixed untreated A549 cells, UV (1 hour) and 100 nM Calyculin A (30 minutes) treated HeLa cells using Phospho-JUN (Ser73) antibody (80086-1-RR, Clone: 4A18) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR004).

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  FC experiment of NIH/3T3 using 80086-1-RR

  1x10^6 NIH/3T3 cells untreated (dashed lines) or treated with UV were intracellularly stained with 0.13 ug Phospho-JUN (Ser73) Recombinant antibody (80086-1-RR, Clone:4A18) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Tested Applications

• Positive WB detected in: UV treated NIH/3T3 cells
• Positive IF/ICC detected in: UV (1 hour) and 100 nM Calyculin A (30 minutes) HeLa cells
• Positive FC (Intra) detected in: UV treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

80086-1-RR targets Phospho-JUN (Ser73) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: jun oncogene
• Calculated Molecular Weight: 331 aa, 36 kDa
• Observed Molecular Weight: 42-45 kDa
• GenBank Accession Number: BC068522
• Gene Symbol: JUN
• Gene ID (NCBI): 3725
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis (PMID: 22180088). JUN is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. JUN is a basic leucine zipper (bZIP) transcription factor that acts as homo- or heterodimer, binding to DNA and regulating gene transcription (PMID: 9732876). In additon, extracellular signals can induce post-translational modifications of JUN, resulting in altered transcriptional activity and target gene expression (PMID:8464713). More over, it has uncovered multiple layers of a complex regulatory scheme in which JUN is able to crosstalk, amplify and integrate different signals for tissue development and disease. Jun is predominantly nuclear, ubiquitinated Jun colocalizes with lysosomal proteins (PMID: 15469925). This antibody is raised against synthetic phosphopeptide corresponding to residues surrounding Ser73 of human JUN, which can detect the bands around 42-45 kDa.

Publications

Species	Application	Title
rat	WB	J Ethnopharmacol; Discovery and validation of anti-arthritic ingredients and mechanisms of Qingfu Juanbi Tang, a Chinese herbal formulation, on rheumatoid arthritis; Authors - Muzi Peng
mouse	WB	Heliyon; LINC02257 regulates colorectal cancer liver metastases through JNK pathway; Authors - Xiangan Wu
		Int J Biol Macromol; Protective effect of ferulic acid-hyaluronic acid copolymer against UVB irradiation in a human HaCaT cell line; Authors - Min Jiang