Filter: AllWBIHC

WB analysis using 60666-1-Ig (same clone as 60666-1-PBS)
Non-treated and UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (HRP-60004) antibody as a loading control. This data was developed using the same antibody clone with 60666-1-PBS in a different storage buffer formulation.
×
Non-treated and UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (HRP-60004) antibody as a loading control. This data was developed using the same antibody clone with 60666-1-PBS in a different storage buffer formulation.
WB analysis using 60666-1-Ig (same clone as 60666-1-PBS)
Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control. This data was developed using the same antibody clone with 60666-1-PBS in a different storage buffer formulation.
×
Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control. This data was developed using the same antibody clone with 60666-1-PBS in a different storage buffer formulation.
WB analysis using 60666-1-Ig (same clone as 60666-1-PBS)
Non-treated and UV treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control. This data was developed using the same antibody clone with 60666-1-PBS in a different storage buffer formulation.
×
Non-treated and UV treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control. This data was developed using the same antibody clone with 60666-1-PBS in a different storage buffer formulation.
WB analysis using 60666-1-Ig (same clone as 60666-1-PBS)
Non-treated and Anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control. This data was developed using the same antibody clone with 60666-1-PBS in a different storage buffer formulation.
×
Non-treated and Anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control. This data was developed using the same antibody clone with 60666-1-PBS in a different storage buffer formulation.
IHC staining of Jurkat using 60666-1-Ig (same clone as 60666-1-PBS)
Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60666-1-PBS in a different storage buffer formulation.

Phospho-JNK (Thr183/Tyr185) Monoclonal antibody, PBS Only

Phospho-JNK (Thr183/Tyr185) Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 60666-1-PBS

Clone No.2H8F12

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

JNK, MAPK8, JNK 2, JNK 1, EC:2.7.11.24

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

60666-1-PBS targets Phospho-JNK (Thr183/Tyr185) in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	mitogen-activated protein kinase 8
Calculated Molecular Weight	48 kDa
Observed Molecular Weight	42 kDa, 50 kDa
GenBank Accession Number	NM_138982
Gene Symbol	JNK
Gene ID (NCBI)	5599
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P45983
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. The antibody can detect endogenous levels of p46 and p54 SAPK/JNK when phosphorylated at Thr183 and Tyr185. It will also react with JNK singly phosphorylated at Thr183.