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Phospho-JNK (Thr183/Tyr185) Monoclonal antibody

Phospho-JNK (Thr183/Tyr185) Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2H8F12

Cat no : 60666-1-Ig

Synonyms

JNK (Thr183/Tyr185), Phospho-JNK, phospho-JNK(Thr183/Tyr185), phospho‐JNK/SAPK (Thr183/Tyr185), phospho-JNK1/2 (Thr183/Tyr185)

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-JNK (Thr183/Tyr185) Monoclonal antibody (60666-1-Ig)

    WB analysis using 60666-1-Ig

    Non-treated and UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (HRP-60004) antibody as a loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-JNK (Thr183/Tyr185) Monoclonal antibody (60666-1-Ig)

    WB analysis using 60666-1-Ig

    Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-JNK (Thr183/Tyr185) Monoclonal antibody (60666-1-Ig)

    WB analysis using 60666-1-Ig

    Non-treated and UV treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-JNK (Thr183/Tyr185) Monoclonal antibody (60666-1-Ig)

    WB analysis using 60666-1-Ig

    Non-treated and Anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control.

  • Immunohistochemistry (IHC) staining of Jurkat cells using Phospho-JNK (Thr183/Tyr185) Monoclonal antibody (60666-1-Ig)

    IHC staining of Jurkat using 60666-1-Ig

    Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, NIH/3T3 cells, HSC-T6 cells, UV treated HEK-293 cells, UV treated NIH/3T3 cells, UV treated HSC-T6 cells, Anisomycin treated HeLa cells
Positive IHC detected inJurkat cells
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60666-1-Ig targets Phospho-JNK (Thr183/Tyr185) in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name mitogen-activated protein kinase 8
Calculated Molecular Weight 48 kDa
Observed Molecular Weight42 kDa, 50 kDa
GenBank Accession NumberNM_138982
Gene Symbol JNK
Gene ID (NCBI) 5599
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. The antibody can detect endogenous levels of p46 and p54 SAPK/JNK when phosphorylated at Thr183 and Tyr185. It will also react with JNK singly phosphorylated at Thr183.

Protocols

Product Specific Protocols
WB protocol for Phospho-JNK (Thr183/Tyr185) antibody 60666-1-IgDownload protocol
IHC protocol for Phospho-JNK (Thr183/Tyr185) antibody 60666-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols