Phospho-INSR (Tyr1150/1151)/IGF1R (Tyr1135/1136) Polyclonal antibody, PBS Only

Phospho-INSR (Tyr1150/1151)/IGF1R (Tyr1135/1136) Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 31133-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HEK-293T using 31133-1-AP (same clone as 31133-1-PBS)

  Non-treated and IGF-1 treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 31133-1-AP (Phospho-INSR (Tyr1150/1151)/IGF1R (Tyr1135/1136) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 31133-1-PBS in a different storage buffer formulation.

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Product Information

31133-1-PBS targets Phospho-INSR (Tyr1150/1151)/IGF1R (Tyr1135/1136) in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: INSR
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: BC117172
• Gene Symbol: INSR
• Gene ID (NCBI): 3643
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Insulin plays a crucial role in brain functions such as memory improvement and energy metabolism. The INSR shares a high structural homology with the IGF1R (84% similarity in the tyrosine kinase domain, 45-65% in the ligand-binding domain, and more than 50% in the overall amino acid sequence). In addition, ligand-dependent activation of the INSR and IGF1R activates almost identical downstream signaling cascades. This antibody recognizes the phosphorylation of tyrosine 1150 and 1151 of INSR, as well as the phosphorylation of tyrosine 1135 and 1136 of IGF1R. ((PMID:24434591))