Phospho-INSR (Tyr1150/1151)/IGF1R (Tyr1135/1136) Polyclonal antibody

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  WB analysis of HEK-293T using 31133-1-AP

  Non-treated and IGF-1 treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 31133-1-AP (Phospho-INSR (Tyr1150/1151)/IGF1R (Tyr1135/1136) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Phospho-INSR (Tyr1150/1151)/IGF1R (Tyr1135/1136) Polyclonal Antibody for WB, ELISA

Cat No. 31133-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Synonyms

CD220, HHF5, INSR, insulin receptor, IR

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Tested Applications

• Positive WB detected in: IGF-1 treated HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

31133-1-AP targets Phospho-INSR (Tyr1150/1151)/IGF1R (Tyr1135/1136) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: INSR
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: BC117172
• Gene Symbol: INSR
• Gene ID (NCBI): 3643
• RRID: AB_3669868
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P06213
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Insulin plays a crucial role in brain functions such as memory improvement and energy metabolism. The INSR shares a high structural homology with the IGF1R (84% similarity in the tyrosine kinase domain, 45-65% in the ligand-binding domain, and more than 50% in the overall amino acid sequence). In addition, ligand-dependent activation of the INSR and IGF1R activates almost identical downstream signaling cascades. This antibody recognizes the phosphorylation of tyrosine 1150 and 1151 of INSR, as well as the phosphorylation of tyrosine 1135 and 1136 of IGF1R. ((PMID:24434591))

Publications

Species	Application	Title
mouse	WB	Nat Commun; Intestinal Nogo-B reduces GLP1 levels by binding to proglucagon on the endoplasmic reticulum to inhibit PCSK1 cleavage; Authors - Ke Gong