Calyculin A treated and untreated HeLa cells were subjected to SDS PAGE followed by western blot with 84739-2-RR (Phospho-Histone H3 (Thr3) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.
Calyculin A treated and untreated HeLa cells were subjected to SDS PAGE followed by western blot with 84739-2-RR (Phospho-Histone H3 (Thr3) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.
FC experiment of NIH/3T3 using 84739-2-RR
1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-Histone H3 (Thr3) Recombinant antibody (84739-2-RR, Clone:241897D9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.
1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-Histone H3 (Thr3) Recombinant antibody (84739-2-RR, Clone:241897D9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
Calyculin A treated and untreated HeLa cells were subjected to SDS PAGE followed by western blot with 84739-2-RR (Phospho-Histone H3 (Thr3) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.
FC (INTRA) Figures
FC experiment of NIH/3T3 using 84739-2-RR
1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-Histone H3 (Thr3) Recombinant antibody (84739-2-RR, Clone:241897D9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Phospho-Histone H3 (Thr3) Recombinant antibody
Catalog Number
84739-2-RR
Citations
-
Dilutions
WB : 1:5000-1:50000 FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
Applications
WB, FC (Intra), ELISA
Reactivity
human, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
