Phospho-EGFR (Tyr869) Recombinant antibody

Phospho-EGFR (Tyr869) Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241785G5

Cat no : 84839-1-RR

Synonyms

EGFR Tyr845, EGFR (Tyr845), p-EGFR, p-EGFR (Tyr845), p-EGFR Tyr845

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Validation Data Gallery

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  WB analysis using 84839-1-RR

  Non-treated and EGF treated A431 cells were subjected to SDS PAGE followed by western blot with 84839-1-RR (Phospho-EGFR (Tyr869) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

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  FC experiment of A431 using 84839-1-RR

  1x10^6 EGF treated A431 cells were intracellularly stained with 0.25 ug Phospho-EGFR (Tyr869) Recombinant Antibody (84839-1-RR, Clone:241785G5) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Tested Applications

• Positive WB detected in: EGF treated A431 cells
• Positive FC (Intra) detected in: EGF treated A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84839-1-RR targets Phospho-EGFR (Tyr869) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Calculated Molecular Weight: 1210 aa, 134 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC094761
• Gene Symbol: EGFR
• Gene ID (NCBI): 1956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Epidermal growth factor receptor (EGFR) is a member of the receptor tyrosine kinase (RTK) family and plays a critical role in a wide variety of cellular functions, including proliferation, differentiation and apoptosis. After EGFR homodimerization with extracellular ligands such as epidermal growth factor (EGF), the intracellular tyrosine kinase domain is activated and autophosphorylates multiple tyrosine residues in the C‐terminal tail domain. (PMID: 23822636) This antibody recognizes EGFR phosphorylation at tyrosine 869, which is also widely described as the tyrosine 845 site.