Phospho-Cardiac Troponin I (Ser23/24) Recombinant antibody

Phospho-Cardiac Troponin I (Ser23/24) Recombinant Antibody for WB, ELISA
Cat No. 85002-1-RR
Clone No.241982B1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Cardiac Troponin I (Ser23/24), P-Cardiac Troponin I (Ser23/24), P-Cardiac Troponin I, Phospho-Cardiac Troponin I, Phospho-Troponin I (Cardiac)

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inmouse heart tissue, λ phosphatase treated mouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85002-1-RR targets Phospho-Cardiac Troponin I (Ser23/24) in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide Predict reactive species
Full Name troponin I type 3 (cardiac)
Calculated Molecular Weight 210 aa, 24 kDa
Observed Molecular Weight26 kDa
GenBank Accession NumberBC096165
Gene Symbol Cardiac Troponin I
Gene ID (NCBI) 7137
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP19429
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cardiac Troponin I (cTnI) is produced by cardiac muscle. To date, most investigative effort in phosphorylation mediated regulation of cTnI function has focused on the actions of PKA and PKC. Evidence from studies in a variety of systems, ranging from reconstituted myofilament proteins to cultured myocytes or transgenic animals with heterologous expression of modified cTnI proteins, indicates that PKA-mediated functional effects (eg, decreased myofilament Ca2 sensitivity) are mediated principally through the phosphorylation of Ser22/23 (equivalent to Ser23/24, including the initiating Met, in the rodent sequence) within the unique N-terminal extension of cTnI. (PMID: 15514163)

Protocols

Product Specific Protocols
WB protocol for Phospho-Cardiac Troponin I (Ser23/24) antibody 85002-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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