Phospho-Cardiac Troponin I (Ser23/24) Recombinant antibody

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  WB analysis using 85002-1-RR

  Non-treated mouse heart tissue, phosphatase inhibitor treated mouse heart tissue and λ phosphatase treated mouse heart tissue were subjected to SDS PAGE followed by western blot with 85001-1-RR (Phospho-Cardiac Troponin I (Ser23/24) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

Phospho-Cardiac Troponin I (Ser23/24) Recombinant Antibody for WB, ELISA

Cat No. 85002-1-RR

Clone No.241982B1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Cardiac Troponin I (Ser23/24), P-Cardiac Troponin I (Ser23/24), P-Cardiac Troponin I, Phospho-Cardiac Troponin I, Phospho-Troponin I (Cardiac)

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Tested Applications

• Positive WB detected in: mouse heart tissue, λ phosphatase treated mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85002-1-RR targets Phospho-Cardiac Troponin I (Ser23/24) in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: troponin I type 3 (cardiac)
• Calculated Molecular Weight: 210 aa, 24 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC096165
• Gene Symbol: Cardiac Troponin I
• Gene ID (NCBI): 7137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P19429
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cardiac Troponin I (cTnI) is produced by cardiac muscle. To date, most investigative effort in phosphorylation mediated regulation of cTnI function has focused on the actions of PKA and PKC. Evidence from studies in a variety of systems, ranging from reconstituted myofilament proteins to cultured myocytes or transgenic animals with heterologous expression of modified cTnI proteins, indicates that PKA-mediated functional effects (eg, decreased myofilament Ca2 sensitivity) are mediated principally through the phosphorylation of Ser22/23 (equivalent to Ser23/24, including the initiating Met, in the rodent sequence) within the unique N-terminal extension of cTnI. (PMID: 15514163)