Phospho-Beta Catenin (Ser33) Recombinant antibody

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  WB analysis using 80067-1-RR

  Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80067-1-RR (Phospho-Beta Catenin (Ser33)antibody) at dilution of 1:10000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with beta tubulin (66240-1-Ig) antibody as loading control.

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  WB analysis using 80067-1-RR

  Non-treated HT-29 and Calyculin A treated treated HT-29 cells were subjected to SDS PAGE followed by western blot with 80067-1-RR (CTNNB1 antibody) at dilution of 1:10000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with beta tubulin (66240-1-Ig) antibody as loading control.

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  FC experiment of PC-3 using 80067-1-RR

  1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.25 ug Anti-Human Phospho-Beta Catenin (Ser33) (80067-1-RR, Clone:3K1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-Beta Catenin (Ser33) Uni-rAb^TM Recombinant Antibody for WB, ELISA, FC (Intra)

Cat No. 80067-1-RR

Clone No.3K1

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, FC (Intra), ELISA

Synonyms

Beta catenin, Catenin beta 1, beta-Catenin, B-catenin, b cat

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Tested Applications

• Positive WB detected in: PC-3 cells, HT-29 cells, Calyculin A treated HT-29 cells, Calyculin A treated PC-3 cells
• Positive FC (Intra) detected in: Calyculin A treated PC-3 cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below

Product Information

80067-1-RR targets Phospho-Beta Catenin (Ser33) in WB, FC (Intra), ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: catenin (cadherin-associated protein), beta 1, 88kDa
• Calculated Molecular Weight: 781 aa, 86 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC058926
• Gene Symbol: Beta Catenin
• Gene ID (NCBI): 1499
• ENSEMBL Gene ID: ENSG00000168036
• RRID: AB_2918861
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P35222
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222). CK1 phosphorylates β-Catenin at Ser45. This phosphorylation event primes β-Catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-Catenin protein levels and have been found in many tumor cell lines .

Publications

Species	Application	Title
mouse	WB	J Ethnopharmacol; Mechanism of Buyang Huanwu Decoction mediating Cav1-regulated Wnt pathway to promote neural regeneration in cerebral ischemic mice; Authors - Yin OuYang
mouse	WB	Mediators Inflamm; Liraglutide Attenuates Hepatic Oxidative Stress, Inflammation, and Apoptosis in Streptozotocin-Induced Diabetic Mice by Modulating the Wnt/β-Catenin Signaling Pathway; Authors - Jie Yu
human	WB	Front Pharmacol; Autophagy Blockade by Ai Du Qing Formula Promotes Chemosensitivity of Breast Cancer Stem Cells Via GRP78/β-Catenin/ABCG2 Axis.; Authors - Mianmian Liao
human	WB	Acta Biochim Biophys Sin (Shanghai); Targeting GRP78 enhances the sensitivity of HOS osteosarcoma cells to pyropheophorbide-α methyl ester-mediated photodynamic therapy via the Wnt/β-catenin signaling pathway.; Authors - Qiang Zuo
mouse	WB	Exp Ther Med; Insulin and liraglutide attenuate brain pathology in diabetic mice by enhancing the Wnt/β-catenin signaling pathway.; Authors - Yuan Zhao
human	WB	Biochem Pharmacol; SMURF1 leads to the β-catenin signaling-mediated progression of esophageal squamous carcinoma by losing PATZ1-induced CCNG2 transcription; Authors - Lingling Chen