Phospho-Beta Catenin (Ser33) Recombinant antibody, PBS Only

Phospho-Beta Catenin (Ser33) Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3K1

Cat no : 80067-1-PBS

Synonyms

Beta catenin, Catenin beta 1, beta-Catenin, B-catenin, b cat

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Validation Data Gallery

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  WB analysis using 80067-1-RR (same clone as 80067-1-PBS)

  Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80067-1-RR (Phospho-Beta Catenin (Ser33)antibody) at dilution of 1:10000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with beta tubulin (66240-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 80067-1-PBS in a different storage buffer formulation.

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  WB analysis using 80067-1-RR (same clone as 80067-1-PBS)

  Non-treated HT-29 and Calyculin A treated treated HT-29 cells were subjected to SDS PAGE followed by western blot with 80067-1-RR (CTNNB1 antibody) at dilution of 1:10000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with beta tubulin (66240-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 80067-1-PBS in a different storage buffer formulation.

• 

  FC experiment of PC-3 using 80067-1-RR (same clone as 80067-1-PBS)

  1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.25 ug Anti-Human Phospho-Beta Catenin (Ser33) (80067-1-RR, Clone:3K1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80067-1-PBS in a different storage buffer formulation.

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Product Information

80067-1-PBS targets Phospho-Beta Catenin (Ser33) in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: catenin (cadherin-associated protein), beta 1, 88kDa
• Calculated Molecular Weight: 781 aa, 86 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC058926
• Gene Symbol: Beta Catenin
• Gene ID (NCBI): 1499
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222). CK1 phosphorylates β-Catenin at Ser45. This phosphorylation event primes β-Catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-Catenin protein levels and have been found in many tumor cell lines .