Phospho-CRKL (Tyr207) Recombinant antibody

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  WB analysis using 84590-1-RR

  Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84590-1-RR (Phospho-CRKL (Tyr207) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

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  FC experiment of K-562 using 84590-1-RR

  1X10^6 K-562 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.06 ug Phospho-CRKL (Tyr207) Recombinant antibody (84590-1-RR, Clone:240881B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-CRKL (Tyr207) {ptg:Brand} Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 84590-1-RR

Clone No.240881B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

p-CRKL (Tyr207), p-CRKL, CRKL (Tyr207), CrkL (phospho Y207), phospho CRKL (Tyr 207)

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, λ phosphatase treated NIH/3T3 cells
• Positive FC (Intra) detected in: λ phosphatase treated K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84590-1-RR targets Phospho-CRKL (Tyr207) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: v-crk sarcoma virus CT10 oncogene homolog (avian)-like
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC043500
• Gene Symbol: CRKL
• Gene ID (NCBI): 1399
• RRID: AB_3672086
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P46109
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Similar to other adaptor proteins, Crk functions primarily to assemble molecular complexes to distribute signals within the cell. The best studied regulatory mechanism of Crk proteins is the phosphorylation at a tyrosine located in a linker between the N- and C-terminal SH3 domains (Tyr221 in human CrkII, Tyr207 in human CrkL). (PMID: 24675776)