Phospho-Beta Catenin (Ser675) Recombinant antibody, PBS Only

Phospho-Beta Catenin (Ser675) Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6D6

Cat no : 80084-1-PBS

Synonyms

b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286

---

Validation Data Gallery

• 

  WB analysis using 80084-1-RR (same clone as 80084-1-PBS)

  Lambda protein phosphatase treated HEK-293 and NIH/3T3 and Non-treated HEK-293 and NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80084-1-RR (Phospho-Beta Catenin (Ser675) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was re-blotted with Beta Catenin antibody 66379-1-Ig as control. This data was developed using the same antibody clone with 80084-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 80084-1-RR (same clone as 80084-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated MCF-7 cells using Phospho-Beta Catenin (Ser675) antibody (80084-1-RR, Clone: 6D6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 80084-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 80084-1-RR (same clone as 80084-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Phospho-Beta Catenin (Ser675) antibody (80084-1-RR, Clone: 6D6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80084-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80084-1-PBS targets Phospho-Beta Catenin (Ser675) in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: catenin (cadherin-associated protein), beta 1, 88kDa
• Calculated Molecular Weight: 781 aa, 86 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC058926
• Gene Symbol: CTNNB1
• Gene ID (NCBI): 1499
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222). PKA was shown to phosphorylate β-catenin at Ser675. Phosphorylation at Ser675 induces β-catenin accumulation in the nucleus and increases its transcriptional activity.