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Phospho-Beta Catenin (Ser675) Recombinant antibody

Phospho-Beta Catenin (Ser675) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

6D6

Cat no : 80084-1-RR

Synonyms

b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-Beta Catenin (Ser675) Recombinant antibody (80084-1-RR)

    WB analysis using 80084-1-RR

    Lambda protein phosphatase treated HEK-293 and NIH/3T3 and Non-treated HEK-293 and NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80084-1-RR (Phospho-Beta Catenin (Ser675) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was re-blotted with Beta Catenin antibody 66379-1-Ig as control.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Phospho-Beta Catenin (Ser675) Recombinant antibody (80084-1-RR)

    IF Staining of MCF-7 using 80084-1-RR

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated MCF-7 cells using Phospho-Beta Catenin (Ser675) antibody (80084-1-RR, Clone: 6D6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Phospho-Beta Catenin (Ser675) Recombinant antibody (80084-1-RR)

    IF Staining of MCF-7 using 80084-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Phospho-Beta Catenin (Ser675) antibody (80084-1-RR, Clone: 6D6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Phospho-Beta Catenin (Ser675) Recombinant antibody (80084-1-RR)

    IF Staining of MCF-7 using 80084-1-RR

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated MCF-7 cells using Phospho-Beta Catenin (Ser675) antibody (80084-1-RR, Clone: 6D6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

Tested Applications

Positive WB detected inHEK-293 cells, λ phosphatase treated HEK-293, λ phosphatase treated NIH/3T3 cells
Positive IF/ICC detected inλ phosphatase treated MCF-7 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

80084-1-RR targets Phospho-Beta Catenin (Ser675) in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Calculated Molecular Weight 781 aa, 86 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC058926
Gene Symbol Beta Catenin
Gene ID (NCBI) 1499
RRIDAB_2918863
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222). PKA was shown to phosphorylate β-catenin at Ser675. Phosphorylation at Ser675 induces β-catenin accumulation in the nucleus and increases its transcriptional activity.

Protocols

Product Specific Protocols
WB protocol for Phospho-Beta Catenin (Ser675) antibody 80084-1-RRDownload protocol
IF protocol for Phospho-Beta Catenin (Ser675) antibody 80084-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Ginsenoside Rc Promotes Bone Formation in Ovariectomy-Induced Osteoporosis In Vivo and Osteogenic Differentiation In Vitro.

Authors - Nan Yang
View Article