Filter: AllWBIHCFC (Intra)Cytometric bead array

WB analysis using 66317-1-Ig (same clone as 66317-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.
WB analysis of rat brain using 66317-1-Ig (same clone as 66317-1-PBS)
rat brain tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.
WB analysis of human colon using 66317-1-Ig (same clone as 66317-1-PBS)
human colon tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.
WB analysis of rat colon using 66317-1-Ig (same clone as 66317-1-PBS)
rat colon tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66317-1-Ig (same clone as 66317-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66317-1-Ig (Peripherin Antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 66317-1-Ig (same clone as 66317-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66317-1-Ig (Peripherin antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 66317-1-Ig (same clone as 66317-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66317-1-Ig (Peripherin antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66317-1-Ig (same clone as 66317-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66317-1-Ig (Peripherin Antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.
FC experiment of PC-12 using 66317-1-Ig (same clone as 66317-1-PBS)
1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Peripherin (66317-1-Ig, Clone:1H10B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.
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1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Peripherin (66317-1-Ig, Clone:1H10B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP51128-2
Cytometric bead array standard curve of MP51128-2, Peripherin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66317-4-PBS. Detection antibody: 66317-1-PBS. Standard:Ag11116. Range: 0.781-100 ng/mL.

Peripherin Monoclonal antibody, PBS Only (Detector)

Peripherin Monoclonal Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 66317-1-PBS

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, pig

Applications

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

PRPH, NEF4, 1H10B3

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66317-1-PBS targets Peripherin as part of a matched antibody pair:

MP51128-2: 66317-4-PBS capture and 66317-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, rat, pig
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	Peripherin fusion protein Ag11116 Predict reactive species
Full Name	peripherin
Calculated Molecular Weight	470 aa, 54 kDa
Observed Molecular Weight	50-54 kDa
GenBank Accession Number	BC032703
Gene Symbol	Peripherin
Gene ID (NCBI)	5630
RRID	AB_2881698
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P41219
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.