Peripherin Monoclonal antibody

Peripherin Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, pig

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1H10B3

Cat no : 66317-1-Ig

Synonyms

PRPH, NEF4, 1H10B3

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Validation Data Gallery

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  WB analysis using 66317-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 66317-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 66317-1-Ig

  human colon tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 66317-1-Ig

  rat colon tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 66317-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66317-1-Ig (Peripherin antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 66317-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66317-1-Ig (Peripherin antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon using 66317-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66317-1-Ig (Peripherin Antibody) at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of human colon using 66317-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66317-1-Ig (Peripherin Antibody) at dilution of 1:1000 (under 10x lens).

• 

  FC experiment of PC-12 using 66317-1-Ig

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Peripherin (66317-1-Ig, Clone:1H10B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: IMR-32 cells, human colon tissue, rat brain tissue, rat colon tissue, mouse colon tissue, rat spinal cord tissue
• Positive IHC detected in: human brain tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66317-1-Ig targets Peripherin in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, rat, pig samples.

• Tested Reactivity: human, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peripherin fusion protein Ag11116
• Full Name: peripherin
• Calculated Molecular Weight: 470 aa, 54 kDa
• Observed Molecular Weight: 50-54 kDa
• GenBank Accession Number: BC032703
• Gene Symbol: Peripherin
• Gene ID (NCBI): 5630
• RRID: AB_2881698
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.