Peripherin Monoclonal antibody
Peripherin Monoclonal Antibody for WB, IHC, ELISA, FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
human, rat, pig
Applications
WB, IHC, ELISA, FC (Intra)
Conjugate
Unconjugated
CloneNo.
1H10B3
Cat no : 66317-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | rat brain tissue, human colon tissue, rat colon tissue |
Positive IHC detected in | human brain tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | PC-12 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66317-1-Ig targets Peripherin in WB, IHC, ELISA, FC (Intra) applications and shows reactivity with human, rat, pig samples.
Tested Reactivity | human, rat, pig |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peripherin fusion protein Ag11116 |
Full Name | peripherin |
Calculated Molecular Weight | 470 aa, 54 kDa |
Observed Molecular Weight | 50-54 kDa |
GenBank Accession Number | BC032703 |
Gene Symbol | Peripherin |
Gene ID (NCBI) | 5630 |
RRID | AB_2881698 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for Peripherin antibody 66317-1-Ig | Download protocol |
IHC protocol for Peripherin antibody 66317-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |