Peripherin Monoclonal antibody

Peripherin Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, pig

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1H10B3

Cat no : 66317-1-Ig

Synonyms

NEF4, Neurofilament 4, Peripherin, PRPH, PRPH1



Tested Applications

Positive WB detected inrat brain tissue, human colon tissue, rat colon tissue
Positive IHC detected inhuman brain tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inPC-12 cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66317-1-Ig targets Peripherin in WB, IHC, FC, ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peripherin fusion protein Ag11116
Full Name peripherin
Calculated molecular weight 470 aa, 54 kDa
Observed molecular weight 50-54 kDa
GenBank accession numberBC032703
Gene symbol PRPH
Gene ID (NCBI) 5630
RRIDAB_2881698
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Peripherin antibody 66317-1-IgDownload protocol
IHC protocol for Peripherin antibody 66317-1-IgDownload protocol
FC protocol for Peripherin antibody 66317-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols