CoraLite® Plus 488-conjugated PYCR2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PYCR2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17146

Synonyms

FLJ54750, P5C reductase 2, P5CR 2, P5CR2, PYCR2

---

Validation Data Gallery

• 

  IF Staining of HeLa using CL488-17146

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PYCR2 antibody (CL488-17146) at dilution of 1:400.

---

Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-17146 targets PYCR2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PYCR2 fusion protein Ag10918
• Full Name: pyrroline-5-carboxylate reductase family, member 2
• Calculated Molecular Weight: 320 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC020553
• Gene Symbol: PYCR2
• Gene ID (NCBI): 29920
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody may have cross reaction to PYCR1 due to the high homology.