CoraLite®594-conjugated PYCR1 Monoclonal antibody

PYCR1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H2G1

Cat no : CL594-66510

Synonyms

P5CR 1, P5CR, P5C reductase 1, P5C, EC:1.5.1.2

---

Validation Data Gallery

• 

  IF Staining of HeLa using CL594-66510

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 PYCR1 antibody (CL594-66510, Clone: 1H2G1 ) at dilution of 1:200.

• 

  FC experiment of HeLa using CL594-66510

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PYCR1 (CL594-66510, Clone:1H2G1) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66510 targets PYCR1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PYCR1 fusion protein Ag17919
• Full Name: pyrroline-5-carboxylate reductase 1
• Calculated Molecular Weight: 319 aa, 33.8 kDa
• Observed Molecular Weight: 33 kDa, 35 kDa
• GenBank Accession Number: BC022244
• Gene Symbol: PYCR1
• Gene ID (NCBI): 5831
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa.