PYCR1 Polyclonal antibody, PBS Only

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  WB analysis of HeLa using 22150-1-AP (same clone as 22150-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 22150-1-AP (PYCR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22150-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 22150-1-AP (same clone as 22150-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22150-1-AP (PYCR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22150-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 22150-1-AP (same clone as 22150-1-PBS)

  IP result of anti-PYCR1 (IP:22150-1-AP, 4ug; Detection:22150-1-AP 1:1000) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 22150-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 22150-1-AP (same clone as 22150-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 22150-1-AP (PYCR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22150-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PYCR1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 22150-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

22150-1-PBS targets PYCR1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PYCR1 fusion protein Ag17745
• Full Name: pyrroline-5-carboxylate reductase 1
• Calculated Molecular Weight: 319 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC022244
• Gene Symbol: PYCR1
• Gene ID (NCBI): 5831
• RRID: AB_11182280
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P32322
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35kd. This antibody may have cross reaction to PYCR2 due to the high homology.