PYCR1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PYCR1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 22150-1-AP

Synonyms

PIG45, P5CR 1, P5CR, P5C reductase 1, P5C

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Validation Data Gallery

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  WB analysis of HeLa using 22150-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22150-1-AP (PYCR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 22150-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22150-1-AP (PYCR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 22150-1-AP

  IP result of anti-PYCR1 (IP:22150-1-AP, 4ug; Detection:22150-1-AP 1:1000) with HeLa cells lysate 3200ug.

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  IF Staining of HeLa using 22150-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 22150-1-AP (PYCR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, COLO 320 cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

22150-1-AP targets PYCR1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PYCR1 fusion protein Ag17745
• Full Name: pyrroline-5-carboxylate reductase 1
• Calculated Molecular Weight: 319 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC022244
• Gene Symbol: PYCR1
• Gene ID (NCBI): 5831
• RRID: AB_11182280
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35kd. This antibody may have cross reaction to PYCR2 due to the high homology.

Publications

Species	Application	Title
human	WB	Nat Metab; Cancer-associated fibroblasts require proline synthesis by PYCR1 for the deposition of pro-tumorigenic extracellular matrix.; Authors - Emily J Kay ; KD Validated