Pentraxin 3 Polyclonal antibody

• Featured Product
• KD/KO Validated

Pentraxin 3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

26

Cat no : 13797-1-AP

Synonyms

PTX3, TNF alpha induced protein 5, Pentraxin-related protein PTX3, Pentraxin related protein PTX3, Pentaxin-related protein PTX3

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Validation Data Gallery

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  WB analysis of HeLa using 13797-1-AP

  WB result of Pentraxin 3 antibody (13797-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Pentraxin 3 transfected HeLa cells.

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  WB analysis using 13797-1-AP

  HUVEC cells lysates were subjected to SDS PAGE followed by western blot with 13797-1-AP (Pentraxin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13797-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13797-1-AP (Pentraxin 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13797-1-AP

  WB result of Pentraxin 3 antibody (13797-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Pentraxin 3 transfected HeLa cells.

• 

  IF Staining of HepG2 using 13797-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Pentraxin 3 antibody (13797-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: HUVEC cells, HeLa cells, HepG2 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 25 publications below
• IHC: See 9 publications below
• IF: See 9 publications below

Product Information

13797-1-AP targets Pentraxin 3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Pentraxin 3 fusion protein Ag4624
• Full Name: pentraxin-related gene, rapidly induced by IL-1 beta
• Calculated Molecular Weight: 381 aa, 42 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC039733
• Gene Symbol: Pentraxin 3/PTX3
• Gene ID (NCBI): 5806
• RRID: AB_2165302
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pentraxin 3 (PTX3), also known as TNF-inducible gene 14 protein (TSG-14), is a member of the pentraxin superfamily which consists of evolutionarily conserved proteins characterized by a structural motif99, the pentraxin domain (PMID: 15771574). Pentraxin 3 can be produced by a variety of cell types including endothelial cells, smooth muscle cells, adipocytes, fibroblasts, mononuclear phagocytes, and dendritic cells, and its expression is induced by primary inflammatory signals, such as IL-1, TNF, and microbial moieties (PMID: 15771574). Pentraxin 3 is an acute-phase glycoprotein that plays a role in regulating innate resistance to pathogens, inflammation, tissue remodeling and repair, female fertility, and cancer (PMID: 29412047). Pentraxin 3 is a biomarker for cardiovascular disease (PMID: 22347626). Increased plasma Pentraxin 3 levels have been found in patients with cardiovascular disorders (PMID: 22347626; 31057548). In WB, there're two major bands, an upper signal at 80-86 kDa and a lower signal at 42 kDa, representing Pentraxin 3 dimers and monomers, respectively (PMID: 18223257).

Publications

Species	Application	Title
human	WB	J Clin Invest; Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer; Authors - Xiaochao Tan
mouse	WB	Sci Adv; Urchin-like ceria nanoparticles for enhanced gene therapy of osteoarthritis; Authors - Haoyi Chen
human	WB	Nat Commun; SH3RF3 promotes breast cancer stem-like properties via JNK activation and PTX3 upregulation.; Authors - Peiyuan Zhang
human	WB	Front Cell Dev Biol; Pentraxin 3 Promotes Glioblastoma Progression by Negative Regulating Cells Autophagy.; Authors - Zeyu Wang
human,mouse	WB	Ecotoxicol Environ Saf; PTX3 alleviates hard metal-induced acute lung injury through potentiating efferocytosis.; Authors - Weiwen Yan
human	WB	J Proteomics; Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation.; Authors - Sun-Xia Chen

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