C-Reactive Protein/CRP Monoclonal antibody

• Featured Product
• KD/KO Validated

C-Reactive Protein/CRP Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

11

CloneNo.

3F10A11

Cat no : 66250-1-Ig

Synonyms

CRP, PTX1, C-reactive protein(1-205), C-Reactive Protein, C reactive protein

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Validation Data Gallery

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  WB analysis of serum from mouse injected with bacteria using 66250-1-Ig

  Serum from mouse injected with bacteria were subjected to SDS PAGE followed by western blot with 66250-1-Ig (C-Reactive Protein/CRP Antibody) at dilution of 1:2000. Horseradish peroxidase conjugated protein A was used for signal developing instead of secondary antibody incubated at room temperature for 1.5 hours.

• 

  WB analysis of serum from mouse injected with bacteria using 66250-1-Ig

  Serum from normal mouse (left) and mouse injected with bacteria (right) were subjected to SDS PAGE followed by western blot with 66250-1-Ig (C-Reactive Protein/CRP Antibody) at dilution of 1:1000. Horseradish peroxidase conjugated protein A was used for signal developing instead of secondary antibody incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 66250-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66250-1-Ig (C-Reactive Protein/CRP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66250-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66250-1-Ig (C-Reactive Protein/CRP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66250-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66250-1-Ig (C-Reactive Protein/CRP Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 66250-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66250-1-Ig (C-Reactive Protein/CRP Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of human liver using 66250-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using 66250-1-Ig (CRP antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: serum from mouse injected with bacteria, pig liver tissue, rat liver tissue, serum from mouse injected with bacteria tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

66250-1-Ig targets C-Reactive Protein/CRP in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: C-Reactive Protein/CRP fusion protein Ag9883
• Full Name: C-reactive protein, pentraxin-related
• Calculated Molecular Weight: 224 aa, 25 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC020766
• Gene Symbol: CRP
• Gene ID (NCBI): 1401
• RRID: AB_2881638
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.

Publications

Species	Application	Title
rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	IF	Front Immunol; Expression and clinical significance of interleukin-6 pathway in cholangiocarcinoma; Authors - Dongqing Gu
human	IHC	J Cell Mol Med; Proteomic studies in VWA1-related neuromyopathy allowed new pathophysiological insights and the definition of blood biomarkers; Authors - Mohammed Athamneh
mouse	WB	Front Immunol; Binding of Macrophage Receptor MARCO, LDL, and LDLR to Disease-Associated Crystalline Structures.; Authors - Anika Alberts
		Sci Rep; A label-free fiber optic SPR biosensor for specific detection of C-reactive protein.; Authors - Wenjia Wang
human	WB	Sci Rep; C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.; Authors - Anika Alberts

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