CRP Polyclonal antibody

CRP Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24175-1-AP

Synonyms

C reactive protein, CRP, PTX1



Tested Applications

Positive WB detected inhuman plasma, serum from mouse injected with bacteria
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

24175-1-AP targets CRP in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRP fusion protein Ag21466
Full Name C-reactive protein, pentraxin-related
Calculated Molecular Weight 224 aa, 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC125135
Gene Symbol CRP
Gene ID (NCBI) 1401
RRIDAB_2879447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.

Protocols

Product Specific Protocols
WB protocol for CRP antibody 24175-1-APDownload protocol
IHC protocol for CRP antibody 24175-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human, mouseWB

J Proteome Res

Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.

Authors - Shizhen Qin
humanWB

J Proteome Res

Measurement of Organ-Specific and Acute-Phase Blood Protein Levels in Early Lyme Disease.

Authors - Yong Zhou
humanWB

Biomed Mater

Reversing cytotoxicity of uric acid by supramolecular encapsulation with acyclic cucurbit[n]uril.

Authors - Lingguang Kong

Exp Ther Med

TLR4 gene in the regulation of periodontitis and its molecular mechanism.

Authors - Weijuan Qi