PTRF Recombinant antibody

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  WB analysis using 82697-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82697-1-RR (PTRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lung using 82697-1-RR

  rat lung tissue were subjected to SDS PAGE followed by western blot with 82697-1-RR (PTRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 82697-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82697-1-RR (PTRF antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 82697-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTRF antibody (82697-1-RR, Clone: 3E15 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 82697-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82697-1-RR against Human PTRF were performed. The affinity constant is 0.303 nM.

PTRF Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 82697-1-RR

Clone No.3E15

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FKSG13, Cavin-1, Cavin 1, 3E15

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Tested Applications

• Positive WB detected in: mouse lung tissue, rat lung tissue, mouse heart tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82697-1-RR targets PTRF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PTRF fusion protein Ag13486
• Full Name: polymerase I and transcript release factor
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC008849
• Gene Symbol: PTRF
• Gene ID (NCBI): 284119
• RRID: AB_3086511
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6NZI2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.