PTRF Recombinant antibody, PBS Only

PTRF Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E15

Cat no : 82697-1-PBS

Synonyms

FKSG13, Cavin-1, Cavin 1, 3E15

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Validation Data Gallery

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  WB analysis using 82697-1-RR (same clone as 82697-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82697-1-RR (PTRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82697-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat lung using 82697-1-RR (same clone as 82697-1-PBS)

  rat lung tissue were subjected to SDS PAGE followed by western blot with 82697-1-RR (PTRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82697-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 82697-1-RR (same clone as 82697-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTRF antibody (82697-1-RR, Clone: 3E15 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82697-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82697-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82697-1-RR against Human PTRF were performed. The affinity constant is 0.303 nM.

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Product Information

82697-1-PBS targets PTRF in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PTRF fusion protein Ag13486
• Full Name: polymerase I and transcript release factor
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC008849
• Gene Symbol: PTRF
• Gene ID (NCBI): 284119
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.