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CoraLite® Plus 488-conjugated PTPN9 Monoclonal antibody

PTPN9 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D6E4

Cat no : CL488-67931

Synonyms

MEG2, PTPaseMEG2, PTPase MEG2, Protein-tyrosine phosphatase MEG2, EC:3.1.3.48

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated PTPN9 Monoclonal ant (CL488-67931)

    IF Staining of U2OS using CL488-67931

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 488 PTPN9 antibody (CL488-67931, Clone: 1D6E4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated PTPN9 Monoclonal ant (CL488-67931)

    IF Staining of U2OS using CL488-67931

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 488 PTPN9 antibody (CL488-67931, Clone: 1D6E4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated PTPN9 Monoclonal ant (CL488-67931)

    FC experiment of MCF-7 using CL488-67931

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PTPN9 (CL488-67931, Clone:1D6E4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67931 targets PTPN9 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PTPN9 fusion protein Ag30615
Full Name protein tyrosine phosphatase, non-receptor type 9
Calculated Molecular Weight 593 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC010863
Gene Symbol PTPN9
Gene ID (NCBI) 5780
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTPN9, Tyrosine-protein phosphatase non-receptor type 9, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN9 is involved in the transfer of hydrophobic ligands or in functions of the Golgi apparatus (PMID: 19167335). MiR-96 inhibits PTPN9 expression and consequently promotes proliferation, migration and invasion of breast cancer cells (PMID:27857177).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PTPN9 antibody CL488-67931Download protocol
FC protocol for CL Plus 488 PTPN9 antibody CL488-67931Download protocol
Standard Protocols
Click here to view our Standard Protocols