PTPN23 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PTPN23 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10472-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of A375 using 10472-1-AP (same clone as 10472-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 10472-1-AP (same clone as 10472-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10472-1-AP (same clone as 10472-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10472-1-AP (same clone as 10472-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 10472-1-AP (same clone as 10472-1-PBS)

  IP result of anti-PTPN23 (IP:10472-1-AP, 5ug; Detection:10472-1-AP 1:500) with HeLa cells lysate 1000ug. This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10472-1-AP (same clone as 10472-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10472-1-AP (PTPN23 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10472-1-AP (same clone as 10472-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10472-1-AP (PTPN23 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 10472-1-AP (same clone as 10472-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10472-1-AP (PTPN23 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 10472-1-AP (same clone as 10472-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PTPN23 antibody (10472-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

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Product Information

10472-1-PBS targets PTPN23 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTPN23 fusion protein Ag0765
• Full Name: protein tyrosine phosphatase, non-receptor type 23
• Calculated Molecular Weight: 179 kDa
• Observed Molecular Weight: 179 kDa
• GenBank Accession Number: BC004881
• Gene Symbol: PTPN23
• Gene ID (NCBI): 25930
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PTPN23(Tyrosine-protein phosphatase non-receptor type 23) is also named as HD-PTP,PTP-TD14 and KIAA1471.It encodes a 185-kDa protein which is expressed in human endothelial fromthe umbilical cord and in human Kaposi-spindle cells(PMID:16408268).PTPN23 is a non-transmembrane protein tyrosine phosphatase which contributes to the signal transduction pathways involved in the regulation of cell migration and invasion(PMID:22510412).