PTPN23 Polyclonal antibody

• Featured Product
• KD/KO Validated

PTPN23 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 10472-1-AP

Synonyms

KIAA1471, His domain-containing protein tyrosine phosphatase, HD-PTP, HDPTP, HD PTP

---

Validation Data Gallery

• 

  WB analysis of A375 using 10472-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 10472-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10472-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10472-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10472-1-AP

  IP result of anti-PTPN23 (IP:10472-1-AP, 5ug; Detection:10472-1-AP 1:500) with HeLa cells lysate 1000ug.

• 

  IHC staining of human colon cancer using 10472-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10472-1-AP (PTPN23 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10472-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10472-1-AP (PTPN23 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10472-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10472-1-AP (PTPN23 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 10472-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PTPN23 antibody (10472-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

---

Tested Applications

• Positive WB detected in: A375 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below

Product Information

10472-1-AP targets PTPN23 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTPN23 fusion protein Ag0765
• Full Name: protein tyrosine phosphatase, non-receptor type 23
• Calculated Molecular Weight: 179 kDa
• Observed Molecular Weight: 179 kDa
• GenBank Accession Number: BC004881
• Gene Symbol: PTPN23
• Gene ID (NCBI): 25930
• RRID: AB_2173382
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPN23(Tyrosine-protein phosphatase non-receptor type 23) is also named as HD-PTP,PTP-TD14 and KIAA1471.It encodes a 185-kDa protein which is expressed in human endothelial fromthe umbilical cord and in human Kaposi-spindle cells(PMID:16408268).PTPN23 is a non-transmembrane protein tyrosine phosphatase which contributes to the signal transduction pathways involved in the regulation of cell migration and invasion(PMID:22510412).

Publications

Species	Application	Title
human	IF	Nat Commun; Concerted ESCRT and clathrin recruitment waves define the timing and morphology of intraluminal vesicle formation.; Authors - Eva Maria Wenzel
human	WB,IHC	Genes Dev; Suppression of protein tyrosine phosphatase N23 predisposes to breast tumorigenesis via activation of FYN kinase.; Authors - Siwei Zhang ; KD Validated
human	IF	EMBO J; ESCRT-mediated lysosome repair precedes lysophagy and promotes cell survival.; Authors - Maja Radulovic
human	WB, IF	Curr Biol; UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.; Authors - Stefani Flavia F
	WB	Curr Biol; Recruitment of UBPY and ESCRT exchange drive HD-PTP-dependent sorting of EGFR to the MVB.; Authors - Nazim Ali ; KD Validated
human	WB	Cell Rep; Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9.; Authors - Jonathan M Goodwin