PTPN22 Polyclonal antibody, PBS Only

PTPN22 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11783-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 11783-1-AP (same clone as 11783-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11783-1-AP (PTPN22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11783-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11783-1-AP (same clone as 11783-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11783-1-AP (PTPN22 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11783-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 11783-1-AP (same clone as 11783-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11783-1-AP (PTPN22 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11783-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Jurkat using 11783-1-AP (same clone as 11783-1-PBS)

  IP result of anti-PTPN22 (IP:11783-1-AP, 4ug; Detection:11783-1-AP 1:300) with Jurkat cells lysate 3080ug. This data was developed using the same antibody clone with 11783-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 11783-1-AP (same clone as 11783-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11783-1-AP (PTPN22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11783-1-PBS in a different storage buffer formulation.

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Product Information

11783-1-PBS targets PTPN22 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTPN22 fusion protein Ag2397
• Full Name: protein tyrosine phosphatase, non-receptor type 22 (lymphoid)
• Calculated Molecular Weight: 82 kDa, 92 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC017785
• Gene Symbol: PTPN22
• Gene ID (NCBI): 26191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PTPN22(Tyrosine-protein phosphatase non-receptor type 22) is also named as LyP,PEP,PTPN8.It belongs to the protein-tyrosine phosphatase family and non-receptor class 4 subfamily.It acts as negative regulator of T-cell receptor (TCR) signaling.Defects in PTPN22 are a cause of susceptibility to systemic lupus erythematosus (SLE). It has some isoforms produced by alternative splicing with calculated molecular mass of 79-92 kDa, and apparent molecular mass of 100-110 kDa.