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CoraLite®555-conjugated SHP2 Monoclonal antibody

SHP2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3F8A8

Cat no : CL555-66795

Synonyms

PTPN11/SHP2, PTPN11, NS1, NS 1, EC:3.1.3.48

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®555-conjugated PTPN11/SHP2 Monoclonal ant (CL555-66795)

    IF Staining of MCF-7 using CL555-66795

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CoraLite®555 PTPN11 antibody (CL555-66795, Clone: 3F8A8 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite®555-conjugated PTPN11/SHP2 Monoclonal ant (CL555-66795)

    FC experiment of MCF-7 using CL555-66795

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human PTPN11 (CL555-66795, Clone:3F8A8) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66795 targets SHP2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SHP2 fusion protein Ag13649
Full Name protein tyrosine phosphatase, non-receptor type 11
Calculated Molecular Weight 597 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC008692
Gene Symbol PTPN11
Gene ID (NCBI) 5781
RRIDAB_2919695
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTPN11 (protein tyrosine phosphatase, non-receptor type 11) is also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, MGC14433 and belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism (PMID: 19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions (PMID: 21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML) and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for CL555 SHP2 antibody CL555-66795Download protocol
FC protocol for CL555 SHP2 antibody CL555-66795Download protocol
Standard Protocols
Click here to view our Standard Protocols