PTEN Recombinant antibody, PBS Only

PTEN Recombinant Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, ELISA

Conjugate

Unconjugated

CloneNo.

240134F7

Cat no : 80718-7-PBS

Synonyms

10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1

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Validation Data Gallery

• 

  IF Staining of HeLa using 80718-7-RR (same clone as 80718-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTEN antibody (80718-7-RR, Clone: 240134F7 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80718-7-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 80718-7-RR (same clone as 80718-7-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PTEN Recombinant antibody (80718-7-RR, Clone:240134F7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80718-7-PBS in a different storage buffer formulation.

• 

  Affinity experiments of 80718-7-RR

  Biolayer interferometry (BLl) kinetic assays of 80718-7-RR against Human PTEN were performed. The affinity constant is below 1 pM.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 80718-7-PBS is PTEN Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PTEN fusion protein Ag17274
• Full Name: phosphatase and tensin homolog
• Calculated Molecular Weight: 47 kDa
• GenBank Accession Number: BC005821
• Gene Symbol: PTEN
• Gene ID (NCBI): 5728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.