PSPC1 Recombinant antibody, PBS Only

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  WB analysis using 84657-4-RR (same clone as 84657-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84657-4-RR (PSPC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84657-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84657-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84657-4-RR against Human PSPC1 were performed. The affinity constant is 0.68 nM.

PSPC1 Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 84657-4-PBS

Clone No.242158G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

PSPC 1, PSP1, 242158G7

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Product Information

84657-4-PBS targets PSPC1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PSPC1 fusion protein Ag10136
• Full Name: paraspeckle component 1
• Calculated Molecular Weight: 523 aa, 59 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC014184
• Gene Symbol: PSPC1
• Gene ID (NCBI): 55269
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q8WXF1
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PSPC1 (paraspeckle component 1), a known paraspeckle biomarker, is a putative transcription factor that belongs to the Drosophila behavior/human splicing (DBHS) family. PSPC1 was recently identified as a contextual determinant of tumor progression in multiple cancer types involving oncogenic reprogramming to switch proapoptotic TGF-β to prometastatic TGF-β via hijacking of Smad2/3 targeting. Upregulated PSPC1 is correlated with advanced tumor stages and poor survival of patients with breast, lung, and liver cancers. Upregulated PSPC1 potentiates expression of mesenchymal markers, EMT transcription factors (EMT-TF), cancer stem-like cell transcription factors (CSC-TF), and c-Myc-related proliferation genes, thus promoting migration, invasion, spheroid formation, tumor formation, and metastasis.