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PSPC1 Recombinant antibody, PBS Only

PSPC1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242158G7

Cat no : 84657-4-PBS

Synonyms

PSPC 1, PSP1, 242158G7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSPC1 Recombinant antibody (84657-4-RR)

    WB analysis using 84657-4-RR (same clone as 84657-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84657-4-RR (PSPC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84657-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84657-4-RR

    Affinity and Kinetic Characterization of 84657-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84657-4-RR against Human PSPC1 were performed. The affinity constant is 0.68 nM.

Product Information

84657-4-PBS targets PSPC1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PSPC1 fusion protein Ag10136
Full Name paraspeckle component 1
Calculated Molecular Weight 523 aa, 59 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC014184
Gene Symbol PSPC1
Gene ID (NCBI) 55269
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PSPC1 (paraspeckle component 1), a known paraspeckle biomarker, is a putative transcription factor that belongs to the Drosophila behavior/human splicing (DBHS) family. PSPC1 was recently identified as a contextual determinant of tumor progression in multiple cancer types involving oncogenic reprogramming to switch proapoptotic TGF-β to prometastatic TGF-β via hijacking of Smad2/3 targeting. Upregulated PSPC1 is correlated with advanced tumor stages and poor survival of patients with breast, lung, and liver cancers. Upregulated PSPC1 potentiates expression of mesenchymal markers, EMT transcription factors (EMT-TF), cancer stem-like cell transcription factors (CSC-TF), and c-Myc-related proliferation genes, thus promoting migration, invasion, spheroid formation, tumor formation, and metastasis.