• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PSPC1 Recombinant antibody

PSPC1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242158G7

Cat no : 84657-4-RR

Synonyms

PSPC 1, PSP1, 242158G7

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PSPC1 Recombinant antibody (84657-4-RR)

    WB analysis using 84657-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84657-4-RR (PSPC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84657-4-RR

    Affinity and Kinetic Characterization of 84657-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84657-4-RR against Human PSPC1 were performed. The affinity constant is 0.68 nM.

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, PC-3 cells, MDA-MB-453 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84657-4-RR targets PSPC1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PSPC1 fusion protein Ag10136
Full Name paraspeckle component 1
Calculated Molecular Weight 523 aa, 59 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC014184
Gene Symbol PSPC1
Gene ID (NCBI) 55269
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSPC1 (paraspeckle component 1), a known paraspeckle biomarker, is a putative transcription factor that belongs to the Drosophila behavior/human splicing (DBHS) family. PSPC1 was recently identified as a contextual determinant of tumor progression in multiple cancer types involving oncogenic reprogramming to switch proapoptotic TGF-β to prometastatic TGF-β via hijacking of Smad2/3 targeting. Upregulated PSPC1 is correlated with advanced tumor stages and poor survival of patients with breast, lung, and liver cancers. Upregulated PSPC1 potentiates expression of mesenchymal markers, EMT transcription factors (EMT-TF), cancer stem-like cell transcription factors (CSC-TF), and c-Myc-related proliferation genes, thus promoting migration, invasion, spheroid formation, tumor formation, and metastasis.

Protocols

Product Specific Protocols
WB protocol for PSPC1 antibody 84657-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols