PSME3 Monoclonal antibody, PBS Only

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  WB analysis using 67629-1-Ig (same clone as 67629-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67629-1-Ig (PSME3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67629-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67629-1-Ig (same clone as 67629-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67629-1-Ig (PSME3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67629-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67629-1-Ig (same clone as 67629-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67629-1-Ig (PSME3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67629-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67629-1-Ig (same clone as 67629-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67629-1-Ig (PSME3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67629-1-PBS in a different storage buffer formulation.

PSME3 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67629-1-PBS

Clone No.2A8E8

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

WB, IHC, ELISA

Synonyms

Ki, Ki nuclear autoantigen, PA28 gamma, PA28G, PA28gamma, PSME3, REG GAMMA

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Product Information

67629-1-PBS targets PSME3 in WB, IHC, ELISA applications and shows reactivity with Human, Rat samples.

• Tested Reactivity: Human, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PSME3 fusion protein Ag6716
• Full Name: proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC001423
• Gene Symbol: PSME3
• Gene ID (NCBI): 10197
• RRID: AB_2882830
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P61289
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

PSME3 gene encodes proteasome activator complex subunit 3, which is also called REG-gamma or PA28-gamma. REG-gamma activates the trypsin-like catalytic subunit of the proteasome thus is a proteasome regulator. MDM2-TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of TP53, may be promoted by REG-gamma resulting in inhibited apoptosis after DNA damage. REG-gamma may also be involved in cell cycle regulation.