PSME1 Monoclonal antibody, PBS Only

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  WB analysis using 67928-1-Ig (same clone as 67928-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67928-1-Ig (PSME1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67928-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67928-1-Ig (same clone as 67928-1-PBS)

  WB result of PSME1 antibody (67928-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSME1 transfected HeLa cells. This data was developed using the same antibody clone with 67928-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67928-1-Ig (same clone as 67928-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PSME1 antibody (67928-1-Ig, Clone: 1F7E2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67928-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PSME1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 67928-1-PBS

Clone No.1F7E2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

REG-alpha, PA28A, Interferon gamma up-regulated I-5111 protein, IGUP I-5111, IFI5111

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Product Information

67928-1-PBS targets PSME1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PSME1 fusion protein Ag30549
• Full Name: proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC007503
• Gene Symbol: PSME1
• Gene ID (NCBI): 5720
• RRID: AB_2918680
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q06323
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The principal function of the proteasome is targeted degradation of intracellular proteins. Activity of the 20S proteasome is controlled by regulatory complexes that bind to the ends of the cylindrical proteasome. 11S regulator (REG or PA28), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. Human PSME1 and PSME2 genes encode the two proteasome activators PA28 alpha and beta, respectively, which have been implicated in antigen processing for loading class I MHC molecules. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.