CoraLite® Plus 488-conjugated PSMD9 Monoclonal antibody

• Featured Product
• KD/KO Validated

PSMD9 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H2G1

Cat no : CL488-67338

Synonyms

p27, PSMD9, Rpn4

---

Validation Data Gallery

• 

  IF Staining of HeLa using CL488-67338

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 PSMD9 antibody (CL488-67338, Clone: 1H2G1 ) at dilution of 1:200.

• 

  IF Staining of U2OS using CL488-67338

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 488 PSMD9 antibody (CL488-67338, Clone: 1H2G1 ) at dilution of 1:200.

---

Tested Applications

• Positive IF/ICC detected in: HeLa cells, U2OS cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67338 targets PSMD9 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PSMD9 fusion protein Ag25654
• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 9
• Calculated Molecular Weight: 27 kDa
• GenBank Accession Number: BC004213
• Gene Symbol: PSMD9
• Gene ID (NCBI): 5715
• RRID: AB_2919454
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PSMD9 is a ubiquitous protein of eukaryotic cells and is a chaperon of the 26S proteasome complex, which degrades ubiquitinated proteins in eukaryotic cells and contributes to the degradation of intracellular proteins into antigenic peptides for antigen presentation by MHC class I cells. The 26S mammalian base sub-complex involves three distinct modules which have ATPase subunits distinctly associated to three chaperones, one of which is PSMD9 regulating the modules assembly. The PSMD9 ubiquitous regulatory role within the proteasome implies its potential pleiotropic effects within different physio-pathological systems. PSMD9 is known to form a stable subcomplex with PSMC3 and PSMC6, two of the AAA-ATPases, assisting in the assembly of the 20S and 19S particles to form the holo complex.